No fractionation is associated with water uptake by roots, a process usually driven by capillary tension, with the one exception of xerophytes that burn ATP to pump water in extremely arid environments ( with a roughly 100 depletion ).
2.
Air pressure, osmotic pressure, and capillary tension also plays a small part in a tree's ability to draw up water, but this alone would only be sufficient to push the column of water to a height of less than ten metres, and trees can grow much higher than that ( over 100 m ).
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